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Article RSE 16083154

Facilitated Electron Hopping in Nanolayer Oxygen- Insensitive Glucose Biosensor for Application in a Complex Matrix

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ChemElectroChem dx.doi.org/10.1002/celc.201600357, vol. 1884, pp. 1884–18892016.

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P. Cristiani (RSE SpA), M. Grattieri (University of Utah), M. Tucci (Università degli studi di Milano), M. Bestetti (Politecnico di Milano), S. Trasatti (Università degli studi di Milano)

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Electrochemical experimental evidence of facilitated electron transfer in a sub-micrometer layer-bylayer self-assembled biosensor is presented. The sensor is and oxygen insensitive and able to measure glucose.

Electrochemical experimental evidence of facilitated electron transfer in a sub-micrometer biosensor is presented. Layer-bylayer self-assembled deposition provides the unique advantage to specifically control the thickness of the biosensors, allowing an oxygen-insensitive device with a film thickness of 70 nanometers to be obtained.

The biosensor is based on a poly(allylamine) osmium redox mediator and glucose 1-oxidase. The immobilized enzyme contributes to the signal generation through the full biosensor thickness, with no loss of “active enzyme” in the outer layer of the biosensor through reaction with oxygen, as reported in the case of thick redox hydrogels.

The application of the biosensor in complex matrices was approached with tests in wastewater. Encapsulation of the biosensor with NafionS membrane ensured the protection of the enzyme molecules from the external environment, allowing the successful application of the sensor in a complex matrix.

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